Functional effects of the antigen glatiramer acetate are complex and tightly associated with its composition
نویسندگان
چکیده
Glatiramer acetate (Copaxone®; GA) is a non-biological complex drug for multiple sclerosis. GA modulated thousands of genes in genome-wide expression studies conducted in THP-1 cells and mouse splenocytes. Comparing GA with differently-manufactured glatiramoid Polimunol (Synthon) in mice yielded hundreds of differentially expressed probesets, including biologically-relevant genes (e.g. Il18, adj p<9e-6) and pathways. In human monocytes, 700+ probesets differed between Polimunol and GA, enriching for 130+ pathways including response to lipopolysaccharide (adj. p<0.006). Key differences were confirmed by qRT-PCR (splenocytes) or proteomics (THP-1). These studies demonstrate the complexity of GA's mechanisms of action, and may help inform therapeutic equivalence assessment.
منابع مشابه
Glatiramer acetate in the treatment of multiple sclerosis
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ورودعنوان ژورنال:
- Journal of Neuroimmunology
دوره 290 شماره
صفحات -
تاریخ انتشار 2016